BioMed Research International / 2019 / Article / Fig 2

Research Article

Keap1 Cystenine 151 as a Potential Target for Artemisitene-Induced Nrf2 Activation

Figure 2

Cysteine 151 is required for ATT-induced modification to Keap1. (a) COS1 cells were transfected with Keap1-expressing plasmid and treated with varying doses of ATT for 16 hours. Immunoblots show Keap1 and GAPDH levels. (d) COS-1 cells were transfected with vectors expressing either mutant Keap1 (lanes 1 to 12) or wild-type (WT) Keap1 (lanes 13–14) and treated with either 7 μM ATT (+) or DMSO (−) for 16 hours. (b-c) Comparison of the expression levels of modified Keap1 and keap1-69KD at different does of ATT. All values represent mean ± SD of three experiments; ; .