Primary Cilia Blockage Promotes the Malignant Behaviors of Hepatocellular Carcinoma via Induction of Autophagy

Figure 1

Serum starvation induces primary ciliogenesis in HCC cells. (a) Representative immunofluorescence images of cilia marker acetylated tubulin in SMMC-7721, Huh7, HCC-LM3, HepG2, MHCC97-H, SK-Hep-1, and Hep3B HCC cell lines cultured in medium with or without serum for 24 h (×200). (b) Length of primary cilia in seven HCC cell lines after serum starvation for 24 h. (c) Expression level of IFT88 in SMMC-7721 and Huh7 cells were determined by western blot analysis after serum starvation for 24 h; the value under the band is the ratio of the blot and normalized to control. .

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