Primary Cilia Blockage Promotes the Malignant Behaviors of Hepatocellular Carcinoma via Induction of Autophagy

Figure 4

Suppression of autophagy compromises the promalignancy effects of primary ciliogenesis blockage in vitro. (a) SMMC-7721 and Huh7 cells were transfected with si-ATG7 and/or si-IFT88 (si-NC as negative control) to inhibit autophagy/primary ciliogenesis for 48 h. Cell viability was measured by CCK-8 assay for another 24 h, 48 h, and 72 h, respectively. (b, c) Migration and invasion ability were measured by transwell assay for another 24 h (×200). , .

(a)

(b)

(c)