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BioMed Research International
Volume 2019, Article ID 5318729, 8 pages
https://doi.org/10.1155/2019/5318729
Research Article

Anti-Invasion and Antimetastatic Effects of Porcine Recombinant NK-lysin on SMMC-7721 Human Hepatocellular Carcinoma Cells

1Laboratory Animal Center, Shanxi Agricultural University, Taigu, Shanxi, China
2College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, China
3Plastic & Reconstructive Surgery, University of Colorado Denver, Denver, CO, USA

Correspondence should be addressed to Junbing Jiang; moc.621@yerffejdnxs

Received 12 January 2019; Revised 28 February 2019; Accepted 20 March 2019; Published 21 April 2019

Academic Editor: Ziad Fajloun

Copyright © 2019 Kuohai Fan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The high invasion and metastasizing abilities of hepatocellular carcinoma (HCC) are the primary reasons for the high mortality rate of patients. Therefore, identification of agents to inhibit invasion and metastasis is very important for treatment of HCC. We analyzed the anti-invasion and antimetastatic effects of porcine recombinant NK-lysin, which was designed and expressed in vitro by our research group, on SMMC-7721 hepatocellular carcinoma cells via wound-healing assays, adhesion assays, invasion assays, real-time polymerase chain reaction (PCR), and Western blot analysis. MTT assay results indicated that NK-lysin inhibited the growth of SMMC-7721 cells in a dose- and time-dependent manner. NK-lysin reduced the ability of cell migration, adhesion, and invasion. Based on gene and protein expression analysis, NK-lysin decreased β-catenin and MMP-2 expression. These results suggested that NK-lysin has anti-invasion and antimetastatic effects on hepatocellular carcinoma cells in vitro by reducing the level of the β-catenin and MMP-2.