Figure 4: Physical interaction of PC1 truncate mutation, PKR, and mTOR in HeLa cells. (a) Interaction between mTOR and PC1-5TMC. 200mg of protein extracted was immunoprecipitated with antibody against GFP (EU4), and 20mg of protein extracted was detected by immunoblotting assays with antibody against GFP (B-2). The blot was reprobed with anti-mTOR. 5TMC, GFP-tagged PC1-5TMC; Ctrl, GFP. (b) Interaction between PKR and PC1-5TMC. Experiments performed are similar to those in panel (a). The blot was reprobed with anti-PKR.