p38/TF/HIF-α Signaling Pathway Participates in the Progression of CIPN in Mice
Figure 1
L-OHP induced mechanical allodynia in mice and enhanced over-expression of TF in sciatic nerve and macrophages. (a) Mechanical thresholds after a single-injection of vehicle or L-OHP (4 mg/kg, i.p.) (n = 6). (b) Mechanical thresholds after four times injection of vehicle or L-OHP (4 mg/kg, i.p., injected on 1, 4, 8 and 11 day) (n = 6). (c) TF expression in the sciatic nerve after a single injection of vehicle or L-OHP (4 mg/kg, i.p.) at 4 and 8 h (n = 4). (d) TF expression in the sciatic nerve after two times injection of vehicle or L-OHP (4 mg/kg, i.p., injected on 1 and 4 day) (n = 4). (e) TF expression in the blood after a single injection of vehicle or L-OHP (4 mg/kg, i.p.) at 4 and 8 h (n = 4). (f) TF expression in the blood after two times injection of vehicle or L-OHP (4 mg/kg, i.p., injected on 1 and 4 day) (n = 4). (g) TF expression in RAW264.7 cells after vehicle or L-OHP (10 μM) administration (n = 4). Significant difference was revealed following one-way or two-way ANOVA (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. control; Bonferroni post hoc tests).