p38/TF/HIF-α Signaling Pathway Participates in the Progression of CIPN in Mice
Figure 4
Hirudin can significantly suppress the overexpression of p-p38, HIF-1α and MMP-9/2 induced by L-OHP and attenuate CIPN. (a) Mechanical thresholds after four times injection of vehicle or L-OHP (4 mg/kg, i.p., injected on 1, 4, 8 and 11 day) (n = 6). (b) The expression of p-p38, p38 and HIF-1α in sciatic nerve of mice on 14 day after four times injection of vehicle or L-OHP (4 mg/kg, i.p., injected on 1, 4, 8 and 11 day) (n = 4). The activity of MMP-9/2 in sciatic nerve (c) and the blood (d) of mice on 14 day after four times injection of vehicle or L-OHP (4 mg/kg, i.p., injected on 1, 4, 8 and 11 day) (n = 4). Hirudin (10 mg/kg, i.p.) was administrated once per day till 14 days. The first administration of hirudin was from the day before the CIPN model was built. Significant difference was revealed following one-way ANOVA (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. L-OHP group; Bonferroni post hoc tests).