Caffeic Acid Phenethyl Ester (CAPE) Induces VEGF Expression and Production in Rat Odontoblastic Cells

Figure 6

VEGF receptor mRNA expressions in CAPE-treated KN-3 cells in normal medium and osteogenic induction medium. KN-3 cells were stimulated with caffeic acid (10 μg/ml), CAPE (10 μg/ml), or EGCG (10 μg/ml) for 6 hours in normal medium or osteogenic induction medium. After stimulation, total RNA was isolated and mRNA expression levels of VEGFR-1 and VEGFR-2 were analyzed by real-time RT-PCR. (a) VEGFR-1 mRNA expression levels in normal medium. (b) VEGFR-1 mRNA expression levels in osteogenic induction medium. (c) VEGFR-2 mRNA expression levels in normal medium. (d) VEGFR-2 mRNA expression levels in osteogenic induction medium. Values represent the means ± SDs representative of three independent experiments, and each experiment was performed in triplicate. Asterisks indicate significant differences versus the control (without polyphenols) ( vs. control).

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