BioMed Research International / 2019 / Article / Fig 2

Research Article

Effects of Different Methionine Sources on Methionine Metabolism in the IPEC-J2 Cells

Figure 2

Effects of different Met sources on the contents of transsulfuration-related metabolites and cofactors (a) and remethylation-related metabolites and cofactors (b) in IPEC-J2 cells. Cells were seeded on cell dishes and cultured in DMEM/F-12 medium for 24 h, then cultured in serum-free medium for 18 h and then in Met-free medium for 6 h, and finally treated with different Met sources in the medium for 2 h. Shown are representative 3 independent experiments. The data are expressed as the mean ± SEM. A, B, C means without the same letter had extremely significant difference, P < 0.01; a, b means without the same letter had significant difference, P < 0.05.

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