Effects of Different Methionine Sources on Methionine Metabolism in the IPEC-J2 Cells
Figure 3
Effects of different Met sources on mRNA expression levels of the key enzymes involved in Met metabolism in IPEC-J2 cells. The mRNA expression of the Met transmethylation relative genes is shown in (a) and (b), the mRNA expression of the Met transsulfuration relative genes is shown in (c) and (d), and the mRNA expression of the Met remethylation relative genes is shown in (e) and (f). Cells were seeded onto six-well Transwell system plates and cultured in DMEM/F-12 medium for 21 d, then cultured in serum-free medium for 18 h and then in Met-free medium for 6 h, and finally treated with different Met sources in the medium for 2 h. Shown are representative 3 independent experiments. The data are expressed as the mean ± SEM. A, B, C, means without the same letter had extremely significant difference, P < 0.01; a, b means without the same letter had significant difference, P < 0.05.