Ligustrazine Prevents Intervertebral Disc Degeneration via Suppression of Aberrant TGFβ Activation in Nucleus Pulposus Cells

Figure 5

The effect of LIG on HNPC in vitro. (a–c) Representative immunostaining images of HNPC treated with 5 ng/mL rmTGFβ1 combined with or without 10⁻⁵ M LIG, or 0.01% DMSO (rmTGFβ1 + Veh) for CCN2 (a), pSmad2/3 (b), and ACAN (c). (d–f) Quantification of CCN2, pSmad2/3, and ACAN expressions in (a)–(c). Each column represents the mean ± SE of three independent experiments. vs Control, vs rmTGFβ.

(a)

(b)

(c)

(d)

(e)

(f)