Microarray Analysis For Expression Profiles of lncRNAs and circRNAs in Rat Liver after Brain-Dead Donor Liver Transplantation

Table 1

Primers designed for qRT-PCR validation of candidate lncRNAs and mRNAs.

Gene	Primer	Annealing temperature (℃)	Product length (bp)
GAPDH (RAT)	F:5′ GCTCTCTGCTCCTCCCTGTTCTA 3′	60	124
	R:5’ TGGTAACCAGGCGTCCGATA 3′
Inhba	F:5′ AACGGGTATGTGGAGATAGA 3′	60	239
	R:5′ TGAAACAGACGGATGGTG 3′
Per2	F:5′ TTTTTCTGCCGTGTCAGTGTT 3′	60	213
	R:5′GTTTGGTGTGTGGGTTGTTGT 3’
Rb1	F:5′ AAATCATCGTAACTGCGTAT3′	60	104
	R:5′GTAGAACACTATAATGGAATCAAAC 3′
Trib3	F:5′ CAAGTTGCGTCGATTTGTC 3′	60	245
	R:5′ CAGAGTCCTGGAACGGGTAT 3′
Hmox1	F:5′ GGTCCTGAAGAAGATTGCG3′	60	258
	R:5′GAGGGACTCTGGTCTTTGTG 3′
Ndrg1	F:5′ CTTCGGCAAGGAGGAGATAC 3′	60	196
	R:5’ TCCAACCACGAGCAGAGC 3’
Angptl4	F:5′ AACGCCACCCGCTTACAC 3′	60	142
	′ AGCCTCCATCTGAAGTCATCTC 3′
Atf3	F:5′ AATTGCTGCTGCCAAGTGT 3′	60	184
	R:5′ CTGAGCCCGGACGATACA 3′
LOC103692832	F:5′ ATGGTTCGGGAGAGTTACTAGC 3′	60	103
	R:5′ ATGGTTCCAGAGGGATAAGAGT 3′
LOC102555648	F:5′ CGATCTAAAACTTGTCCGAACA 3′	60	87
	R:5′ CAGCTACTAAACCCGTCCGT 3′
LOC102554711	F:5′ AATGACGGATGAGCCGATAC 3′	60	66
	R:5′ CGCCCAAGTAGTTGTTGCA 3′
Ablim3-XR_596974	F:5′ TACCGCTCAGGTGATTTGTCC 3′	60	205
	R:5′ GCATACTGCGGTCAAAATCG 3′
Ablim3-XR_596973	F:5′ TACTACCGCTCAGCTGGAGAAA 3′	60	99
	R:5′ TTCACTCGTCTTGCTCTTGGTT 3′
LOC103692056	F:5′ TGGACACTGGGGAGACATT 3′	60	175
	R:5′ AAACTGCTCCATGATTGCTG 3′