Casein Kinase 2 Interacting Protein-1 Suppresses Glioma Cell Proliferation via Regulating the AKT/GSK3/-Catenin Pathway

Figure 2

Altered CKIP-1 expression affected glioma cell proliferation. (a) Expression of CKIP-1 mRNA in four glioma cell lines. GAPDH was the loading control. (b) Overexpression of CKIP-1 in U251 cells by flag-tagged vector pcDNA3.1 for CKIP-1 (U251-CKIP-1) or empty vehicle (U251-Ctrl). (c) Cell proliferation was determined by Cell Counting Kit-8 (CCK-8) assay every day for 5 days. (d) Cell proliferation was assessed by 5′-bromodeoxyuridine (BrdU) incorporation 24 h and 96 h after transfection of glioma cells with vehicle (Ctrl), CKIP-1 (e). Cell growth of U251-Ctrl cells was assayed every day for 5 days. (f) Colony formation assay and quantification of U251 cells with CKIP-1 overexpression. p < 0.05, p < 0.01, vs. control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)