Casein Kinase 2 Interacting Protein-1 Suppresses Glioma Cell Proliferation via Regulating the AKT/GSK3/-Catenin Pathway

Figure 3

Antiproliferative and proapoptotic activity of CKIP-1 in glioma cells. (a) Cell cycle distributions were detected followed by propidium iodide (PI) staining in U251 cells transfected with CKIP-1 for 72 h by flow cytometry. (b) Cell cycle assays of percentage of cells in G₀/G₁ and S phases were quantified. (c) Annexin V-FITC/PI staining was analyzed from cells mentioned above. Lower right quadrant represents Annexin V⁺/PI^- cells (early apoptotic cells). Upper right quadrant shows Annexin V⁺/PI⁺ cells (late apoptotic cells). (d) Apoptosis assay of percentages of apoptotic (early and late apoptotic) cells was quantified.

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