Casein Kinase 2 Interacting Protein-1 Suppresses Glioma Cell Proliferation via Regulating the AKT/GSK3/-Catenin Pathway

Figure 4

CKIP-1 inhibited the AKT/GSK3β/β-catenin pathway in U251 cells. (a-b) Western blot analysis for total Akt, p-Akt (T308), p-Akt (S473), total GSK3β, p-GSK3β (S9), p-β-catenin (S33/37), β-catenin (nucleus), β-catenin, cyclin D1, and c-myc protein expression in U251, U251-NC, and U251-CKIP-1 cells, respectively. NC: negative control. p < 0.05 vs. U251, ^# p < 0.05 vs. U251-NC. (c) IHC scores of CKIP-1 and AKT/GSK3β/β-catenin expression in normal human or glioma patients’ brain tissues. p < 0.05 vs. normal brain tissue.