L-Carnitine Reduces Oxidative Stress and Promotes Cells Differentiation and Bone Matrix Proteins Expression in Human Osteoblast-Like Cells

Figure 3

L-Carnitine (L-C) effect on intracellular calcium pathway. (a): Representative Western blot and relevant quantification of CaMKIIα phosphorylation in hOBs treated with 5 mM L-C. CaMKIIα activation was observed at 5 and 15 min. (b): Immunofluorescence assay and quantification of total CaMKII protein after 6 and 24h. L-C enhanced the protein content of total CaMKII compared to controls after 6h. CaMKII (red), nuclei (DAPI), and merge. The quantified signal was normalized for the total nuclei number (magnification: 40X). Data are the mean ± SD of six experiments performed with cells obtained from different donors. For immunofluorescence and Western blot analyses: Student’s t test: <0.05 vs control (ctrl).

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