Alleviation of Cartilage Destruction by Sinapic Acid in Experimental Osteoarthritis

Table 1

Gene specific primer sequences qPCR.

Genes	qRT-PCR primers
IL-1β	Forward: ATGGCAGAAGTACCTAAGCTCGC
IL-1β	Reverse: ACACAAATTGCATGGTGAAGTCAGTT
IL-6	Forward: ACACACTGGTTCTGAGGGAC
IL-6	Reverse: TACCACAAGGTTGGCAGGTG
TNF-α	Forward: ATGAGCACAGAAAGCATGATCCGC
TNF-α	Reverse: CCAAAGTAGACCTGCCCGGACTC
MMP-1	Forward: GCCACAAAGTTGATGCAGTT
MMP-1	Reverse: GCAGTTGAACCAGCTATTAG
MMP-3	Forward: ATGAAAATGAAGGGTCTTCCGG
MMP-3	Forward: GCAGAAGCTCCATACCAGCA
MMP-13	Forward: ATGCATTCAGCTATCCTGGCCA
MMP-13	Reverse: AAGATTGCATTTCTCGGAGCCTG
ADAMTS4	Forward: ATGGCCTCAATCCATCCCAG
ADAMTS4	Reverse: AAGCAGGGTTGGAATCTTTGC
ADAMTS5	Forward: GGAGCGAGGCCATTTACAAC
ADAMTS5	Reverse: CGTAGACAAGGTAGCCCACTTT
ACTB	Forward: TGACGGGGTCACCCACACTGTGCCCATCTA
ACTB	Reverse: CTAGAAGCATTTGCGGTGGACGATGGAGGG
HO-1	Forward: ACATCGACAGCCCCACCAAGTTCAA
HO-1	Reverse: CTGACGAAGTGACGCCATCTGTGAG

IL: interleukin; TNF: tumor necrosis factor; MMP: matrix metalloproteinase; ADAMTS: a disintegrin and metalloproteinase with thrombospondin motif; qRT-PCR: quantitative real-time polymerase chain reaction; ACTB: Actin; HO: heme oxygenase.