BioMed Research International / 2019 / Article / Tab 6 / Research Article
Snapshot of Phylogenetic Groups, Virulence, and Resistance Markers in Escherichia coli Uropathogenic Strains Isolated from Outpatients with Urinary Tract Infections in Bucharest, Romania Table 6 Primers sequences used in simplex and multiplex PCR assays for virulence genes.
The gene Primer Amplification size and Tm References chuA F: 5′-GACGAACCAACGGTCAGGAT-3′ R: 5′-TGCCGCCAGTACCAAAGACA-3′ 279 bp (multiplex) 55°C Clermont et al., 2000 yjaA F: 5′-TGAAGTGTCAGGAGACGCTG-3′ R: 5′-ATGGAGAATGCGTTCCTCAAC-3′ 211bp (multiplex) 55°C Clermont et al., 2000 TspE4C2 F: 5′-GAGTAATGTCGGGGCATTCA-3′ R:5′-CGCGCCAACAAAGTATTACG-3′ 152 bp (multiplex) 55°C Clermont et al., 2000 hlyD F: 5′- CTCCGGTACGTGAAAAGGAC-3′ R: 5′-GCCCTGATTACTGAAGCCTG-3′ 904 bp 55°C Rodrigues-Sike et al., 2005 kpsMTII F: 5′- GCG CAT TTG CTG ATA CTG TTG-3′ R: 5′-CAT CAG ACG ATA AGC ATG AGC A-3′ 272 bp 60°C Johnson et al., 2005 [43 ] hlyA F:5′-AACAAGGATAAGCACTGT TCTGGC T-3′ R:5′-ACCATATAAGCGGTCATT CCC GTC A-3′ 1,177 bp 60°C Yamamoto et al., 1995 [44 ] sfaD/E F:5′-CGGAGGAGTAATTACAAACCTGGCA -3′ R: 5′- CTCCGGAGAACTGGGTG ATCTTA C-3′ 408 bp 60°C Blanco et al., 1997 [45 ] fimH F: 5′-TGC AGA ACG GAT AAG CCG TGG -3′ R: 5′- GCA GTC ACC TGC CCT CCG GTA -3′ 508 bp 63°C Rodrigues-Sike et al., 2005 afaBC F: 5′-GCTGGGCAGCAAACTGATAACTCTC -3′ R:5′CATCAAGCTGTTTGTTCGTCCGCCG-3 793 bp 63°C Blanco et al., 1997 cnf-1 F: 5′- GAA CTT ATT AAG GAT AGT-3′ R: 5′-CAT TAT TTA TAA CGC TG-3′ 543kb 40°C Blanco et al., 1997
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6 is reproduced from Grosu et al. 2017 [under the Creative Commons Attribution License/public domain].