Splenectomy Promotes Macrophage Polarization in a Mouse Model of Concanavalin A- (ConA-) Induced Liver Fibrosis

Figure 2

Morphologic features, histological and immunohistochemical staining (magnification ×200), and alanine (ALT) and aspartate aminotransferase (AST) levels in liver tissues from mice in four treatment groups. (a) Gross liver tissues. (b) The levels of ALT and AST detected by enzyme-linked immunosorbent assay. (c) Hematoxylin and eosin (HE) revealing inflammatory cell infiltration in the portal area. (d) Immunohistochemistry used to detect α-smooth muscle actin (SMA) expression in liver tissues. (e) Numbers of activated hepatic stellate cells (HSC) evaluated (immunohistochemical stain). (f) Masson trichrome stain. Blue areas indicate collagen fiber deposition in the liver tissues. (g) Ishak scores. indicates P < 0.05; indicates P < 0.001.

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