A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium/Chitosan/Collagen/β-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice

Figure 1

MSC-CM/hydrogel possesses thermosensitivity, with good biocompatibility and low cytotoxicity. (a) MSC-CM/hydrogel in the liquid phase at 4°C and (b) MSC-CM/hydrogel in the gel phase at 37°C. (c) A viscosity-time curve of the MSC-CM/hydrogel after transfer from 4°C to 37°C. (d) Bright-field images of MSCs cultured in MSC-CM/hydrogel on days 1 (I, II), 3 (III, IV), 5 (V, VI), and 7 (VII, VIII). Bars in I, III, V, and VII represent 2.0 mm and those in II, IV, VI, and VIII represent 500 μm. (e) Assessment of biocompatibility by performing CCK-8 assays; the figure indicates absolute OD values from the CCK-8 assay performed on MSCs cultured with the MSC-CM/hydrogel and under normal culture conditions on days 1, 3, 5, and 7. The asterisk () indicates between MSCs + hydrogel and MSCs control groups at different time points.