MAPK Pathway Inhibitors Attenuated Hydrogen Peroxide Induced Damage in Neural Cells

Figure 2

H₂O₂-induced ROS production, cell apoptosis, and cell cycle change by flow cytometry. (a) The ROS positive cells were determined using ROS-sensitive fluorometric probe. (c) The cell apoptosis and necrosis were measured by Annexin V and PI straining. (e) The cell cycle change was calculated by PI straining. The percentage of cell portions was further shown in subfigures (b) ROS production, (d) cell apoptosis and necrosis, and (f) cell cycle changes, respectively (n=10,000 cells in all experiments).