Research Article
Loratadine, an H1 Antihistamine, Inhibits Melanogenesis in Human Melanocytes
Figure 3
The effects of loratadine on melanogenesis-related signaling pathways. After excluding the influence of PMA by 3 hr starvation, NHM were treated with 7.5 μM loratadine for the indicated times. (a) Whole-cell lysates were analyzed by western blot using antibodies, β-catenin, phospho-Erk, phospho-Akt, and phospho-GSK-3β, and equal protein loading was confirmed by the β-actin levels. Phospho-Akt and phospho-GSK-3β were consistently elevated by loratadine. (b) LY294002, a selective inhibitor of PI3K, could reverse the increase of phospho-Akt in loratadine treated NHM. (c) Loratadine treatment reduced the amount of PKC-βII in the membrane fraction, thereby decreasing its activity.
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