FBW7 Regulates the Autophagy Signal in Mesangial Cells Induced by High Glucose

Figure 4

Effects of overexpression of FWB7 on mTOR/autophagy signal, inflammation, and fibrosis factors. (a) Autolysosome detected by confocal laser scanning microscope. (c) Quantitative analyses of number of autolysosomes. (b) FBW7, p-mTOR, mTOR, and LC3B detected by western blot. (d-f) The corresponding relative gray value statistics graph of the protein level. (g-i) The expression of inflammatory and fibrotic factors in renal mesangial cells detected by ELIS. (g) IL-1β; (h) caspase-1; (i) Col-I. P < 0.05 and P < 0.01.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)