Circulating Exosomes Derived-miR-146a from Systemic Lupus Erythematosus Patients Regulates Senescence of Mesenchymal Stem Cells

Figure 1

SLE serum stimulation promoted senescence of MSCs. (a, b) Cells were cultured in serum from normal persons and SLE patients. BM-MSCs were fixed and stained with SA-β-gal. The number of SA-β-gal-positive cells was increased in the serum-treated normal BM-MSCs in comparison with the normal group. (c) Immunofluorescence showed that the normal distribution of F-actin in the BM-MSCs from normal persons was disordered after being stimulated with SLE serum. (d, e) Western blotting analysis was performed to detect the protein expressions of p16, p27, and p53 (^∗P < 0.05 compared with the normal group, ^#P < 0.05 compared with the normal group, ^&P < 0.05 compared with the normal group) (NOR: normal MSCs group, NOR serum: normal MSCs treated with normal human serum, SLE serum: normal MSCs treated with serum from SLE patients, SLE: SLE MSCs group) ( SLE MSCs: positive control).