Circulating Exosomes Derived-miR-146a from Systemic Lupus Erythematosus Patients Regulates Senescence of Mesenchymal Stem Cells

Figure 5

The schematic mechanism of exosomal miR-146a from SLE patients regulating senescence of MSCs through targeting TRAF6/NF-κB signaling. BM-MSCs were isolated from bone marrow of healthy donors and SLE patients. Serum-derived exosomes were purified by ExoQuick method and cocultured with BM-MSCs for 24h; then we analyzed the senescent status of BM-MSCs, including SA-β-gal staining, the F-actin distribution, and the expression levels of cell cycle-related proteins. miR-146a levels in SLE exosomes significantly declined in comparison with NOR group. miR-146a might negatively regulate MSCs senescence by suppressing TRAF6/NF-κB signaling pathway activation.