Validation of Reference Genes for Gene Expression Normalization in RAW264.7 Cells under Different Conditions

Figure 3

Determination of the optimal number of reference genes for normalization using geNorm analysis. Pairwise variation (Vn/n+1) of reference genes under different conditions are listed. WT, H50 (H100, H200), L0.1 (L0.5, L1), C50 (C100, C200), HG50 (HG100, HG200), and PA50 (PA100, PA200), respectively, were the abbreviation for the control group; H₂O₂, 50 μM (100 μM, 200 μM); lipopolysaccharide, 0.1 μg/mL (0.5, 1 μg/mL); CoCl₂, 50 μM (100 μM, 200 μM); high-glucose, 50 mM (100 mM, 200 mM); and palmitic acid, 50 μM (100 μM, 200 μM).