Validation of Reference Genes for Gene Expression Normalization in RAW264.7 Cells under Different Conditions

Table 1

Information of the ten reference genes used in the real time quantitative PCR.

Gene	Description	Primer: forward/reverse (5’-3’)	Length (bp)	Accession number
ACTB	β-actin	F: CAGGTCATCACTATTGGCAA	143	NM_007393
		R: AGGTCTTTACGGATGTCAAC
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	F: TGCTGAGTATGTCGTGGAGT	136	NM_001289726
		R:GTTCACACCCATCACAAACA
RPL4	Ribosomal protein L4	F: GGAAGTTGGATGAGCTGTAT	108	NM_024212
		R: TCAAGATTCTGCTAAGGTCG
HPRT1	Hypoxanthine phosphoribosyltransferase-1	F: TAGTGAAACTGGAAAAGCCA	135	NM_013556
		R: AAGCTTTACTAGGCAGATGG
PPIA	Cyclophilin A	F:CGGTTCCCAGTTTTTTATCT	102	NM_008907
		R: ATGGCTTCCACAATGTTCAT
CYC1	Cytochrome c-1	F: CTAACCCTGAGGCTGCAAGA	113	NM_025567
		R: GCCAGTGAGCAGGGAAAATA
HMBS	Hydroxymethyl-bilane synthase	F: ATGGCTCAGATAGCATGCAA	126	NM_013551
		R: GGGCTCCTCTTGGAATGTTA
Eef1a1	Eukaryotic translation elongation factor 1 alpha 1	F: CCTACCACCAACTCGTCCAA	136	NM_010106
		R: AAAGGTAACCACCATGCCAG
GUSB	Glucuronidase β	F: ATGTCCTGCTGAGAGGTGTC	121	NM_010368
		R: TCCAGCCTCTCACCAGTAGC
LDHA	lactate dehydrogenase A	F: AACATCTCACTCCCCACAGC	128	NM_010699
		R: CTCACAGGGGTAATCGAAGC