Validation of Reference Genes for Gene Expression Normalization in RAW264.7 Cells under Different Conditions

Table 2

Expression stability values of ten reference genes in RAW264.7 cells calculated by NormFinder analysis.

Rank

WT

HG; 50 mM

HG; 100 mM

HG; 200 mM

H2O2; 50 μM

H2O2; 100 μM

H2O2; 200 μM

LPS; 0.1 μg/mL

LPS; 0.5 μg/mL

LPS; 1 μg/mL

CoCl2; 50 μM

CoCl2; 100 μM

CoCl2; 200 μM

PA; 50 μM

PA; 100 μM

PA; 200 μM

1

RPL4; 0.048

EFl1a1; 0.073

EFl1a1; 0.067

GAPDH; 0.039

RPL4; 0.051

RPL4; 0.043

LDHA; 0.053

LDHA; 0.046

EFl1a1; 0.046

LDHA; 0.056

LDHA; 0.048

LDHA; 0.058

EFl1a1; 0.069

EFl1a1; 0.058

RPL4; 0.081

GUSB; 0.042

2

LDHA; 0.046

GUSB; 0.057

RPL4; 0.042

PPIA; 0.037

LDHA; 0.049

EFl1a1; 0.040

EFl1a1; 0.050

PPIA; 0.036

PPIA; 0.043

GUSB; 0.050

PPIA; 0.048

RPL4; 0.043

LDHA; 0.065

GUSB; 0.048

GUSB; 0.049

PPIA; 0.034

3

PPIA; 0.030

RPL4; 0.053

LDHA; 0.037

EFl1a1; 0.036

PPIA; 0.042

LDHA; 0.039

PPIA; 0.047

GUSB; 0.035

LDHA; 0.040

EFl1a1; 0.049

RPL4; 0.045

GUSB; 0.041

GUSB; 0.048

LDHA; 0.044

EFl1a1; 0.046

EFl1a1; 0.034

4

EFl1a1; 0.027

PPIA; 0.041

GUSB; 0.036

RPL4; 0.031

HPRT1; 0.029

ACTB; 0.035

RPL4; 0.033

ACTB; 0.030

ACTB; 0.036

PPIA; 0.048

ACTB; 0.031

ACTB; 0.027

PPIA; 0.046

RPL4; 0.034

ACTB; 0.040

LDHA; 0.028

5

HPRT1; 0.024

HPRT1; 0.040

GAPDH; 0.031

LDHA; 0.027

EFl1a1; 0.025

PPIA; 0.025

GAPDH; 0.027

EFl1a1; 0.027

GUSB; 0.036

GAPDH; 0.036

GAPDH; 0.029

CYC1; 0.026

RPL4; 0.039

CYC1; 0.032

PPIA; 0.040

ACTB; 0.028

6

ACTB; 0.023

GAPDH; 0.039

ACTB; 0.028

CYC1; 0.020

GUSB; 0.023

GUSB; 0.016

ACTB; 0.023

RPL4; 0.022

RPL4; 0.026

RPL4; 0.032

EFl1a1; 0.028

PPIA; 0.024

HPRT1; 0.028

PPIA; 0.031

HPRT1; 0.039

GAPDH; 0.024

7

GUSB; 0.023

LDHA; 0.035

HMBS; 0.026

HPRT1; 0.020

GAPDH; 0.017

CYC1; 0.016

CYC1; 0.022

CYC1; 0.016

GAPDH; 0.022

ACTB; 0.013

GUSB; 0.027

GAPDH; 0.021

GAPDH; 0.022

ACTB; 0.030

LDHA; 0.034

RPL4; 0.021

8

CYC1; 0.016

HMBS; 0.030

CYC1; 0.025

HMBS; 0.020

HMBS; 0.014

HMBS; 0.015

GUSB; 0.014

HPRT1; 0.012

CYC1; 0.020

CYC1; 0.006

HPRT1; 0.022

EFl1a1; 0.019

CYC1; 0.018

GAPDH; 0.024

CYC1; 0.029

HMBS; 0.020

9

HMBS; 0.015

ACTB; 0.019

HPRT1; 0.023

GUSB; 0.020

CYC1; 0.012

GAPDH; 0.012

HPRT1; 0.008

HMBS; 0.010

HMBS; 0.019

HMBS; 0.006

HMBS; 0.014

HPRT1; 0.015

ACTB; 0.017

HMBS; 0.022

HMBS; 0.029

CYC1; 0.015

10

GAPDH; 0.011

CYC1; 0.018

PPIA; 0.020

ACTB; 0.018

ACTB; 0.010

HPRT1; 0.009

HMBS; 0.006

GAPDH; 0.010

HPRT1; 0.010

HPRT1; 0.002

CYC1; 0.007

HMBS; 0.001

HMBS; 0.006

HPRT1; 0.019

GAPDH; 0.020

HPRT1; 0.015