Tazarotene-Induced Gene 1 (TIG1) Interacts with Serine Protease Inhibitor Kazal-Type 2 (SPINK2) to Inhibit Cellular Invasion of Testicular Carcinoma Cells

Figure 2

TIG1 and SPINK2 suppress cell migration and invasion. NT2/D1 cells were plated in triplicate in 24-well plates. After cells were transfected with TIG1-myc-his and SPINK2-flag expression vectors for 24 h, cells were serum starved for 12 h and were then incubated in medium containing 1% FBS for 24 h. Cell viability was determined by a WST-1 assay (a). NT2/D1 cells were transfected with the indicated expression vectors for 24 h. After serum starvation for 12 h, cells were added in triplicate into the upper transwell insert. Culture medium containing 20% FBS was used as a chemoattractant in the lower wells. Migratory cells (b), and invading cells (c) were stained with PI after 24 h incubation. Representative results from three independent experiments are shown, and the data are presented as the mean ± SD.^∗.

(a)

(b)

(c)