Tazarotene-Induced Gene 1 (TIG1) Interacts with Serine Protease Inhibitor Kazal-Type 2 (SPINK2) to Inhibit Cellular Invasion of Testicular Carcinoma Cells

Figure 3

TIG1 associates with SPINK2. Cell lysates were prepared from NT2/D1 cells transfected with TIG1-myc-his and SPINK2-flag expression vectors for 24 h. The interaction between TIG1-MYC and SPINK2-FLAG was analyzed by coimmunoprecipitation and western blot analysis using anti-MYC or anti-FLAG antibodies (a). Cell lysates were prepared from NT2/D1 cells, and the interaction between TIG1 and SPINK2 was analyzed by coimmunoprecipitation using anti-TIG1 or anti-SPINK2 antibodies followed by western blot analysis (b).

(a)

(b)