Tazarotene-Induced Gene 1 (TIG1) Interacts with Serine Protease Inhibitor Kazal-Type 2 (SPINK2) to Inhibit Cellular Invasion of Testicular Carcinoma Cells

Figure 4

TIG1 co-localizes with SPINK2. NT2/D1 cells were cotransfected with TIG1-myc-his and SPINK2-flag expression vectors for 24 h. After fixation with 4% paraformaldehyde and permeabilization, cells were incubated with anti-MYC and anti-FLAG antibodies followed by incubation with an Alexa Fluor 488-conjugated goat anti-rabbit IgG or an Alexa Fluor 633-conjugated goat anti-mouse IgG antibody. After washing with 1x PBS, cells were stained with DAPI and were then analyzed for TIG1-MYC and SPINK2-FLAG expression with a confocal microscope. Scale bar: 10 μm.