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BioMed Research International
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2019
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Article
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Tab 2
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Research Article
Isoleucine Regulates the Synthesis of Pancreatic Enzymes via the Activation of mRNA Expression and Phosphorylation in the Mammalian Target of Rapamycin Signalling Pathways in Pancreatic Tissues
Table 2
Real-time PCR primers.
Gene name
Reference sequence
Primer sequence (5′-3′)
Product size (bp)
Annealing temperature (°C)
Amylase
NM_001035016
F: GAAATGGCCGTGTGACAGAATTTA
142
64.3
R: ACAAAGACAAGTGCCCTGTCAGAA
Trypsin
NM_001113727
F: TGTCTGCGGCTCACTGCTAC
119
62.7
R: GCTGGGATGGACGATACTCTTG
Chymotrypsin
NM_001098965.1
F: ATGTTGGGCATCACGGTCTT
172
60.0
R: TGTGCCTCCACGTGTTATCC
Lipase
NM_001205820
F: GTGGAAGCAAATGATGGACAAG
81
61.8
R: TGGGTTGAGGGTGAGCAGA
β
-Actin
AF481159
F: ACCACTGGCATTGTCATGGACTCT
152
60.0
R: TCCTTGATGTCACGGACGATTTCC