Isoleucine Regulates the Synthesis of Pancreatic Enzymes via the Activation of mRNA Expression and Phosphorylation in the Mammalian Target of Rapamycin Signalling Pathways in Pancreatic Tissues

Table 2

Real-time PCR primers.

Gene name	Reference sequence	Primer sequence (5′-3′)	Product size (bp)	Annealing temperature (°C)
Amylase	NM_001035016	F: GAAATGGCCGTGTGACAGAATTTA	142	64.3
		R: ACAAAGACAAGTGCCCTGTCAGAA
Trypsin	NM_001113727	F: TGTCTGCGGCTCACTGCTAC	119	62.7
		R: GCTGGGATGGACGATACTCTTG
Chymotrypsin	NM_001098965.1	F: ATGTTGGGCATCACGGTCTT	172	60.0
		R: TGTGCCTCCACGTGTTATCC
Lipase	NM_001205820	F: GTGGAAGCAAATGATGGACAAG	81	61.8
		R: TGGGTTGAGGGTGAGCAGA
β-Actin	AF481159	F: ACCACTGGCATTGTCATGGACTCT	152	60.0
		R: TCCTTGATGTCACGGACGATTTCC