Isoleucine Regulates the Synthesis of Pancreatic Enzymes via the Activation of mRNA Expression and Phosphorylation in the Mammalian Target of Rapamycin Signalling Pathways in Pancreatic Tissues
Table 3
The effects of isoleucine on the activities of enzymes in vitro.
Item
Level of isoleucine (mM1)
SEM2
P-value
0
0.40
0.80
1.60
Tissue concentration (U/g)
ɑ-amylase
814
923
4207
3631
244.7
< 0.001
Trypsin
4.45
5.13
4.87
4.91
0.445
0.148
Chymotrypsin
3.51
4.12
4.32
4.28
0.629
0.093
Lipase
587
634
608
616
88.6
0.347
Release (U/g tissue)
ɑ-amylase
325
308
876
804
48.3
< 0.001
Trypsin
1.92
2.14
2.06
2.26
0.318
0.271
Chymotrypsin
1.22
1.56
1.48
1.49
0.475
0.248
Lipase
189
195
213
204
49.7
0.418
Total activity (U/g tissue)
ɑ-amylase
1139
1231
5083
4435
138.1
< 0.001
Trypsin
6.37
7.27
6.93
7.17
0.582
0.014
Chymotrypsin
4.73
5.68
5.80
5.77
0.536
0.028
Lipase
776
819
821
820
72.3
0.303
One-way ANOVA. Differences were considered significant at P < 0.05. 2Pooled standard error of the means, n=3.