Figure 1: HFDPC expressed mRNA and proteins for several histamine receptor subtypes. HFDPC lysates were prepared and analyzed for alkaline phosphatase activity. Representative results from triplicate samples are presented as the mean ± SD (a). RNA from HFDPC was collected, and the expression levels of the histamine receptor subtypes were analyzed using real-time RT-PCR. After normalizing to β-actin, the relative expression levels are shown (b). HFDPC lysates were prepared, and the expression levels of histamine H1 and H2 receptors were determined by western blot analysis using H1 and H2 antibodies, respectively (c).