Figure 3: Ebastine enhanced the expression of cell cycle regulatory proteins in HFDPC. HFDPC were plated in a 10 cm dish for 24 h. The cells were serum starved for 18 h and then treated with the indicated concentrations of ebastine for 24 h. Cell lysates were prepared, and the expression levels of Cyclin D1, Cyclin E1, Cyclin A, Cdk4, Cdk2, Cdc2, and Actin were determined by immunoblotting (a). The experimental results are presented as the mean (±SD) percentage of the expression level of the indicated cell cycle regulatory proteins normalized to the expression level of the Actin protein in three independent experiments (b). Flow cytometric analysis of cell cycle parameters following 24 h of treatment with ebastine compared with untreated cells (c).