Figure 4: Ebastine affected apoptosis-related proteins in HFDPC. HFDPC were plated in a 10 cm dish for 24 h. The cells were serum starved for 18 h and then treated with the indicated concentrations of ebastine for 24 h. Cell lysates were prepared, and the expression levels of Bcl-2, Bax, and Actin were determined by immunoblotting (a). The experimental results are presented as the mean (±SD) percentage of the expression level of Bcl-2 and Bax proteins normalized to the expression level of the Actin protein in three independent experiments (b).