Figure 5: Ebastine increased p-AKT and p-p44/p42 ERK in HFDPC. HFDPC were plated in a 10-cm dish for 24 h. The cells were serum starved for 18 h and then treated with the indicated concentrations of ebastine for 24 h. Cell lysates were prepared, and the expression levels of p-AKT, total AKT, p- p44/p42 ERK, total p44/42 ERK, and Actin were determined by immunoblotting (a). The experimental results are summarized as the mean (±SD) percentage of the expression level of p-AKT and p-p44/p42 ERK proteins normalized to the expression level of Actin protein in three independent experiments (b).