BioMed Research International / 2019 / Article / Fig 4

Research Article

MiR-455-5p Suppresses the Progression of Prostate Cancer by Targeting CCR5

Figure 4

Regulation of cell proliferation and apoptosis by miR-455-5p through directly targeting CCR5. (a) Protein levels of CCR5 in LNCAP and DU145 cells 72 h after transfection. (b–d) Cell proliferation was assessed by CCK-8 and colony-formation assays in LNCAP and DU145 cells after cotransfection. (e) Cell apoptosis was analyzed by annexin V-FITC plus PI staining in LNCAP and DU145 cells. (f) Western blot revealed the protein levels of caspase 3 and cleaved caspase 3 and CCR5 in LNCAP and DU145 cells 72 h after cotransfection. (g) Representative images of IHC for CCR5 on xenografts. Brown signals on the cell membrane represented CCR5 expression level (Magnification, ×200 bar). (h) Inverse correlation of CCR5 and miR-455-5p in the GSE20132 database (r = −0.28, P = 0.0025). (i) Kaplan–Meier curves for the RFS of patients with PCa stratified by tissue CCR5 levels. , P < 0.05.

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