Identification of a New Serine Alkaline Peptidase from the Moderately Halophilic Virgibacillus natechei sp. nov., Strain FarD^T and its Application as Bioadditive for Peptide Synthesis and Laundry Detergent Formulations

Figure 3

Nucleotide and deduced amino acid sequences of the sapV gene (GenBank accession number: MN094794). The sapV consisted of 1158-bp encoding a polypeptide of 386 amino acid residues. Translation starts at a nucleotide position 1. The first amino acid of the mature protease, Glu, is counted as +1. Numbers written on both sides of the lines indicate the positions of nucleotides and amino acids. The nucleotide sequences ATG and TAA (both highlighted) represent the initiation and terminal codon of translation, respectively. The positions of the four used foreword (F-MS40 and F-MS42) and reverse (R-MS41 and R-MS43) primers were underlined. The black box stands for the NH₂-terminal amino acid sequence of the purified SAPV. SD: Shine–Dalgarno-like sequence. SPR: signal peptide recognition site. SR: stacking region.