Evaluation of Automatic Blood Analyzer as Screening Method in Fetomaternal Hemorrhage

Figure 1

Flow cytometry-based analysis of blood samples, showing the gating strategy. The figure shows a representative sample at 1/10 dilution of umbilical cord blood in blood of male adult. (a) Total blood cells were gated on logarithmic dot plot; (b) nucleated cells were excluded by gating the propidium iodide (PI) negative cell population. (c) Different red blood cell (RBC) populations were detected and quantified based on their fetal hemoglobin content. In addition to histogram, events within the gate of fetal RBCs were also evaluated by plotting the fluorescein isothiocyanate (FITC) channel against phycoerythrin (PE) and side-scatter (SSC) channels to confirm the percentage of events.

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