Evaluation of Automatic Blood Analyzer as Screening Method in Fetomaternal Hemorrhage

Table 2

Comparison between flow cytometry (FCM) and automatic blood analyzer (ABA) for screening of fetal maternal hemorrhage (FMH) in puerperal women (n=40). Thresholds were specified for FCM and ABA following the assessment of blood samples of male adults added with fetal blood. Blood samples were prepared by serially diluting umbilical cord blood in blood of male adults at dilutions 1/10, 1/100, 1/1,000, and 1/10,000.

	All dilutions				1:10				1:100				1:1.000				1:10.000
		ABA				ABA				ABA				ABA				ABA
	Threshold	< 0.500	≥ 0.500	Total	Threshold	< 2.500	≥ 2.500	Total	Threshold	<0.500	≥ 0.500	Total	Threshold	< 2.500	≥ 2.500	Total	Threshold	< 0.500	≥ 0.500	Total
FCM	< 0,0945	27	11	38	< 3,020	32	8	40	< 0,450	29	11	40	< 0,096	30	8	38	< 0,061	22	11	33
	≥ 0,0945	2	0	2	≥ 3,020	0	0	0	≥ 0,450	0	0	0	≥ 0,096	2	0	2	≥ 0,061	7	0	7
	Total	29	11	40	Total	32	8	40	Total	29	11	40	Total	32	8	40	Total	29	11	40