Inhibition of the Nrf2-TrxR Axis Sensitizes the Drug-Resistant Chronic Myelogenous Leukemia Cell Line K562/G01 to Imatinib Treatments
Imatinib-resistant K562/G01 cells had higher levels of Nrf2 expression than the parental K562 cells. (a) The expression levels of Nrf2 mRNA in K562/G01 cells and K562 cells were quantitated by RT-qPCR. (b, c) The expression levels of Nrf2 protein in K562/G01 cells and K562 cells were evaluated by western blot assays. The representative images showed the bands of Nrf2 protein, and β-actin was taken as an internal control (b). The relative expression level of Nrf2 protein was quantitated by calculating the densitometry of targeted bands (c). n = 3 for each group; , compared between the indicated two cell lines.