BioMed Research International / 2019 / Article / Fig 3

Research Article

Inhibition of the Nrf2-TrxR Axis Sensitizes the Drug-Resistant Chronic Myelogenous Leukemia Cell Line K562/G01 to Imatinib Treatments

Figure 3

Identification of a positive correlation between Nrf2 expression and TrxR expression in K562/G01 cells. (a, b) The expression levels of Nrf2 mRNA (a) and TrxR mRNA (b) were quantitated in the indicated cells by RT-qPCR. K562/G01, uninfected blank K562/G01 cells; NC-GFP-LV, NC-GFP-LV lentivirus-infected stable K562/G01 cells; Nrf2-RNAi-LV, Nrf2-RNAi-LV lentivirus-infected stable K562/G01 cells. (c–e) The expression levels of Nrf2 protein and TrxR protein were quantitated in the indicated cells by western blot. The representative images showed the bands of targeted proteins, and β-actin was taken as an internal control (c). The relative expression levels of Nrf2 protein (d) and TrxR protein (e) were quantitated by calculating the densitometry of targeted bands. n = 3 for each group; , compared with the blank K562/G01 cells group.
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