Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model

Figure 1

These images show the making process of fracture model and repetitive brief ischemia. (a) A sterilized 0.8 mm steel K-wire (Zimmer, USA) was used to enter the medullary cavity through the cortical bone by touching the leading edge of the tibial plateau when the keen was bent 90°. The wire stabbed directly the skin and was driven into medullary canal up to the distal of tibia. (b)-(c) X-ray examinations were performed to document the fracture reduction and the position of the implant ((b) anterior-posterior view; (c) lateral view). (d)-(e) Color doppler ultrasound (Vevo2100 imaging system, Canada) confirmed tourniquet pressure selected to block the blood ((d) tourniquet inflated; (e) tourniquet deflated).