Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model

Figure 3

ROI bone tissue image. Region of interest (ROI) was 5mm above and below fracture line; in addition, image-pro plus 6.0 analyzed the grayscale value of bone and callus and modified the color. Green represented callus and red was bone. It can be seen from each image that repeated brief ischemia can significantly increase callus formation.