Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model
Figure 8
The HE dye and BMP-2, VEGF, TGF-β, and ALP immunohistochemical stain results. (a)-(h) HE stains and (i)-(n) immunohistochemical stains. (a)-(b) HE staining of fracture in early stage of rats that received repetitive brief ischemia. Visible callus formation can be seen in the pictures. (c)-(d) HE staining of fracture in early stage of control group rats. The amount of callus is significantly less than that of rats that received repetitive brief ischemia. (e)-(f) HE staining 4 weeks after fracture of rats that received repetitive brief ischemia. It can be seen that the callus has obvious ossification. (g)-(h) HE staining 4 weeks after fracture of control groups rats. The images showed that the degree of ossification in callus was significantly less that of rats that received repetitive brief ischemia. (i)-(j) Immunohistochemical stain of fracture in early stage of rats that received repetitive brief ischemia. A large number of cytokines synthetized can be seen at the fracture site ((i) BMP-2; (j) VEGF). (k)-(l) Immunohistochemical stain of fracture in early stage of control group rats. The synthesis of cytokines is less than that of rats that received repetitive brief ischemia ((k) BMP-2; (l) VEGF). (m)-(n) Immunohistochemical stain 4 weeks after fracture of rats that received repetitive brief ischemia. It can be seen that a large number of ossifications at the callus and the cytokines synthetized have been reduced ((m) TGF-β; (n) ALP). (o)-(p) Immunohistochemical stain 4 weeks after fracture of control group rats. Less ossification at the callus compared to rats that received repetitive brief ischemia and cytokines synthesized also less ((m) TGF-β; (n) ALP).