Antinociceptive Activity of Petroleum Ether Fraction of Clinacanthus nutans Leaves Methanolic Extract: Roles of Nonopioid Pain Modulatory Systems and Potassium Channels

Figure 6

Effect of glibenclamide, apamin, charybdotoxin, and tetraethylammonium chloride on PECN–induced antinociception in the acetic acid–induced abdominal writhing test in mice. Animals were pretreated with glibenclamide (GLIB; 10 mg/kg, i.p.), apamin (APA; 0.04 mg/kg, i.p.), charybdotoxin (CHAR; 0.02, i.p.), or tetraethylammonium chloride (TEA; 4 mg/kg, i.p.) 15 min before oral administration of either vehicle (10 mL/kg) or PECN (500 mg/kg). Each column represents the mean ± SD of six mice. Statistical analyses were performed using 1–way ANOVA followed by Bonferroni’s post hoc test. < 0.001 significantly differed when compared to control group. < 0.001 significantly differed when compared to 500 mg/kg PECN–treated group.