TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway
TRPV1 promoted CRC cell apoptosis through activating p53. HCT116 cells were incubated with capsaicin (50 μM) in the absence or presence of Pifithrin-α (20 μM). (a) Cell viability was determined by CCK-8 assay following indicated treatment. (b) Cell apoptosis were detected by flow cytometry. (c) The expression levels of apoptosis-related protein were examined by Western blotting. (d) The relative intensity was shown as a bar graph. The result was shown as means ± standard deviation. p < 0.05 and p < 0.01 versus control group. ##p < 0.01 and #p < 0.05 versus capsaicin group.
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