TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway
TRPV1 induced cell apoptosis through activating calcineurin. HCT116 cells were incubated with capsaicin (50 μM) in the absence or presence of FK506 (4 μM). (a) The expression level of NFAT2 protein was examined by immunofluorescence assay; images were observed by fluorescence microscopy (magnification, ×400). (b) Cell apoptosis were detected by flow cytometry. (c) The expression levels of apoptosis-related protein were examined by Western blotting. (d) The relative intensity was shown as a bar graph. The result was shown as means ± standard deviation. p < 0.05 and p < 0.01 versus control group. ##p < 0.01 and #p < 0.05 versus capsaicin group.
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